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FAQs on Hormonal Manipulation as an Aquacultural Technique

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Induced spawning in Grey mullet  07/02/05 I am keen to find a copy of a paper by:  C.M. Kuo, C.E. Nash & Z.H. Shehadeh 1974  "A Procedural Guide to Induce Spawning in Grey Mullet".   <I met Nash and Shehadeh in the early seventies in HI... I worked on the hormonal manipulation of Mugil cephalus as an aquacultural technique> I immigrated to Australia in 1979 and currently reside in Inverloch Victoria.  I have an interest in breeding grey mullet on a year round basis.  One application for this technology is to supply the D.P.I. (Department of Primary Industries) with fingerlings to aid in the cleaning up of dairy waste.  I recently downloaded an article by Christopher J. McDonough (Fecundity & spawning season of Striped Mullet) and the above paper was listed as a reference.  However;  I have been unable to obtain a copy.   I am traveling to The States in September and will call in to the Oceanic Institute in Hawaii to meet with C.S. Lee on my way back to Australia.  Dr. Lee is involved with the reproduction of mullet there and has made time available for me to see his operation but my time with him would be much more productive if I were able to read the procedural guide beforehand.  Can you help? <In what way? There are copyright laws re such, but universities may provide such a copy service legally. Bob Fenner> Bibliography Request Dear Bob Please send me a copy of your bibliography for your article:                   A Review of the Literature On Hormonal Manipulation of Fishes As An         Aquacultural Technique                                                     Reading your article got me wondering about the possibility of using crude pituitary extract to induce ovarian growth in the brown treesnake. We are trying to develop methods for reproductive inhibition for this biologically invasive transplant to Guam. Your article does not give information on the number of pituitaries needed to induce reproduction in say, one female fish. Is there a ballpark figure? <Highly dependent of the maturation of the donor/s and stage of development of the adenohypophysis... best to make replicate tests> For fish, do you know whether any success has been obtained using pituitaries from non-reproductive adult females? My problem is that brown treesnakes are seasonal breeders and thus it is very difficult to collect large numbers of reproductive females. <Not nearly as effective to use immature or physiologically inactive females> Thanks for you help and insights!  Tom Mathies Tom Mathies National Wildlife Research Center 4101 LaPorte Avenue Fort Collins, CO  80521-2154 <Biblio. (though dated) on its way to you today. Bob Fenner>

Bibliography hi, Can i have a copy of your bibliography mention in your article Hormonal Manipulation of Fishes As An Aquacultural Technique. Thank you! Regards, Lionel <Yes. Where would you like it mailed to? Bob Fenner>

Re: A sent Bibliography Hi Bob, thank u very much for your kindness! I have received your mail. I really appreciate your help. Feel free to contact mi if u need my help. Many many thanks. Sincerely Lionel <You are welcome my friend. Good luck in your studies, endeavors. Bob Fenner>

Re: Bibliography hi Bob, I really sorry to trouble you again but I really need your help. Hope that you can assist me. I need to find the literature on "Scott, D.B.C., and J.D. Fuller. 1976. The reproductive biology of Scleropages formosus (Muller and Schlegel)(Osteoglossomorpha, Osteoglossidae) in Malaya, and the morphology of its pituitary gland. J.F ish. Biol. (1976) 8, 45-53." mentioned in the bibliography. I had tried to search for it on the website and at our local libraries but could not get it. Your kind assistance will be greatly appreciated. Really thank you very much. Sincerely, Lionel <You'll have to look elsewhere, someone who has access, is located near a large/college library with a life science dept. I make it to such about once a month but am short on time before going on another month long trip soon. Bob Fenner>

How to get my hands on breeding hormones! Well hello Mr. Fenner, I have a short question regarding where I could buy  breeding hormones for fish.  Online would be preferable, a link would be good. Thank You <Mmm... as you can appreciate I am leery of responding w/o asking for clarification. What is your intention? Are you a student, engaged in research? What species, hormones are you interested in? Bob Fenner>

Clarification/sorry (re hormones, breeding) I'm sorry about that, but I figured that a online store would have a wide selection of hormones and I would be able to find what I was looking for by way of browsing.  and yes I'm a student doing an experiment on induced sexual dimorphism.  you also asked me what species I was asking about, well the species I was asking about, was  Barbus titteya (cherry barb).  if it is possible to get  hold of the selected species hormones, I would like to know where and how. once again I'm so sorry to waist your time though thank you for any  (if any) information you might give! oh I almost forgot! I attached a picture of the species thanks for your time. <Ahh, no worries, and much relieved to see you have a "legitimate" interest and purpose. As far as I'm aware (but my info., experience is very dated) there are no "species specific" hormones that can be bought as in from a medical supply house... other than PMSG (from horses), HCG... other human source materials. However, I would make use of BIOSIS and other computer bibliographic tools at the library and contact recent researchers in the field and ask for their assistance in procuring them... Is this clear? Bob Fenner>

Removal of pheromones Dear Robert, Your different paper are very interesting. I work in a seawater recirculating plant to rear a flatfish, the TURBOT ''Scopthalmus maximus''. You often speak about the pheromone, the alarm substance, or other molecule that can have an inhibiting effect on the growth of fish. <Yes> Do you know if ozone can remove or break this molecule, and restore good quality for the growth of the fish ? <I have heard ozone airstripping advanced as a means of removing pheromones, as well as foam fractionation/protein skimming, water changes/flushing. Bob Fenner> Best regard. Yves-Marie MORVAN

RE : Removal of pheromones Dear Robert, Thank you for your quick answer. Do you know if there is a specialist about the pheromone and the removal or the destruction of them ? <Would have to consult the scientific literature, perhaps visit colleges. My work in the field is 25 years old> Do you know where I can find more information about that ? <Through a literature search. As in here: http://www.wetwebmedia.com/litsrchart.htm> Do you know what sort of molecule is it : steroid, protein, other ? <Can be these or other organic molecules: e.g. short-chain fatty acids> Best regard. Yves-Marie MORVAN <Bob Fenner>

Citation info. (not the clunker car) hi Bob have used  your following  writing in my paper. as i found it on web i don't know the exact name of the place it has been published. as a reference i have to mention about the journal, seminar or any place it is related to and the pages and the other possible information. please let me know about the subject:Fenner,B.1976.A review of the literature on hormonal manipulation of fishes as an aquacultural technique. regards Reza <This was written as a graduate seminar paper for a college course and not published in a journal, magazine, book... You may refer folks to WetWebMedia.com as a current (recorded) source. Bob Fenner>  

Induced Breeding I have a question that I am hoping you can help me out with. I am interested in feed breeding from a pet hobbyist point of view. Often I can raise many more fish than I can get from one spawning - will hormone injections allow me to increase the number of offspring or frequency of spawnings?  Do both males and females need to be injected? <Can be either or both. Please read here: http://www.wetwebmedia.com/hormmanfsh.htm> On another note, I breed cichlids.  Do you think that the use of hormone injections would induce fish to interbreed which wouldn't normally breed at all - that is, fish that are separated by special differences based on behavior or coloration could be induced to mate and produce young (probably sterile young - but oh well). <I strongly discourage this (interbreeding) practice. Better for all to keep species separated> This is very important to me- thanks for getting back as soon as possible. Dominic <Bob Fenner>

Re: Induced Breeding Bob, <Dominic> Thank you for your quick reply. The reason I wanted to know about the cichlid interbreeding wasn't for the purposes of hobby keeping, I'm sorry I didn't make that clear.  I'm a card carrying environmentalist - and I care deeply about preserving genetic diversity, and creating monster hybrids was not my goal. <Our values are confluent> The reason I need to know about the interbreeding is for my scientific endeavors.  I am interested in the differences of traits and behaviors between different (but closely related) cichlid species.  The best way to study this is through Quantitative Trait Loci analysis, but in order to perform this experiment one must create hybrids (an F1) generation and study them to determine the genetic location of the genes involved in patterning the traits one is interested in. <There is a bit about this in the scientific literature. Am sure you are aware> So, knowing that I'm not out to make monsters, but to benefit our fishy friends - do you think that use of hormones could overcome the species barriers to create F1's in related cichlid species.  How far do you think it could be pushed?   <Yes, could be done with not much trouble. My MSc. work was involved in such issues (Hormonal manipulation as an aquacultural technique) with Mullets (Mugil cephalus)> I bet that all of the species in Lake Malawi and Victoria could interbreed, based on strict chromosome number comparisons and arrangements - but it is their differences which matter and finding these out requires the experiments that I propose. <Don't think all, but many have been crossed unintentionally by aquarists> If you don't know for sure - could you point me in the right direction to someone who might? <What specifically are you seeking? Broodstock? Technique input? I would do a thorough search of what is known currently first here. Bob Fenner> Thanks again, you're a great help! Dominic

Definition of reproduction terms dear Mr. fenner i am a university student and this is the  second time i write for you. now i have another question and hope receive your scientific and kind answer: what is the distinctive boarder between "maturation" and ripening" in fish gonads. for example, if we have a gonad at first stages of development what can we say to it? how can we know that it belongs to a matured fish  or not? <These terms are easily confused. A "mature" gonad is one of a certain degree of development (capable of producing gametes) whereas a ripe gonad is one that is ready to release eggs or sperm. Bob Fenner> truly Reza

Stages of reproductive development dear bob thanks for your reply, but i didn't get the  answer of my main question. I repeat again and hope receive your answer: if we have a fish gonad at the first stage of development, how can we know that this is belong to a mature fish or not? <... you can section and stain it... look through a microscope for a histological exam...> or which kind of differences may have mature fish gonad at the first steps of development  and immature fish gonads? <Mmm, this touches on the topic of my masters thesis (Hormonal Manipulation of Mullets as an Aquacultural Technique. I am attaching a section of a book I'm penning on ornamental marine fish production. Please read through this and the citations therein. Bob Fenner> regards Reza  

Sperm in Mugil cephalus In the name of God Dear, Dr, Foster&Smith <Actually, we're WetWebMedia. Dr.s F and S are sponsors of our sites> Hello, My name is Sakineh Yeganeh, Mastering of Fisheries engineering in Tehran University My thesis about the effect of extenders in motility of Mugil cephalus spermatozoa. <Ahh, I "worked" on/with this species (Mullet) for several years back in the 1970's> I need some papers about this subject for example structure and biology of Mugil spermatozoa and some factors affecting on motility of spermatozoa specially in Mugil cephalus and other fishes. <Much likely to have been written in the intervening years> If it is possible for you Please help and send this papers for me rapidly. <We're not a research institute... but an (in desire) encyclopedic outlet for practical husbandry of ornamental aquatics. I encourage you to write, request help from colleges and universities about in the world (Mugil cephalus is studied, cultured in many places) that specialize in fisheries. A search on the Net under the terms Fisheries Biology Schools will identify who, where to write> I wait for your sending. Thanks a lot. Best wishes. My address: Karage, Faculty of Agricultural and natural Resources in Tehran University, Department of Fisheries, Sakineh Yeganeh (MS). Please Respond yours sincerely: Yeganeh <Good luck to you my friend. Bob Fenner>

reference request Dear Fenner Robert, I read your article entitled with "manipulation of fishes as an aquacultural technique". It was interesting and indeed I found some useful information. <Though somewhat dated of course> I am here to kindly request you to provide me full reference for "Yamamoto et. al (1966)", cited within sub section "Temperature". Your assistance is highly appreciated. <Yamamoto, K.Y., Nagahama, Y., & F. Yamazaki, 1966. A method to induce articificial spawning of goldfish all through the year. Bull. Jap. Soc. Sci. Fish. Vol. 32 pp. 977-983> with kind regards, Kifle <You're welcome. Bob Fenner> =============================================== Kifle G. Merid Uppsala University, EBC Department of Environmental Toxicology Norbyv?en 18 A, S-752 36 Uppsala, Sweden Tel: 46 018 471 2621 ===============================================

Hormonal Manipulation of Frogs I don?t know if you have the way to send me information of hormonal manipulation of frogs. Hoping to ear from you soon, and thanking in advance. Yours. Daniel.- <Never worked on Amphibians, but on Mullets (Mugil cephalus)... and this decades back... am sure endocrine manipulation has progressed beyond my work. Do have my bibliography (on fish) that can mail. Bob Fenner>

Reference Needed Hello! I am Lim Wei Seng. I would like to ask you whether you can send me the list of reference for the article in the following web address: http://www.wetwebmedia.com/hormmanfsh.htm Thank you very much >> <Please bear with me, and keep after this "project"... and I'll get to keying it onto the site hopefully within a/the week. Bob Fenner... Actually, who would rather mail a copy (made pre-computers)... so do send your SASE for about four ounces and I'll mail you a copy>

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