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FAQs about the Brooklynellosis Disease and Clownfishes, Diagnosis/Symptomology

Related FAQs: Brooklynellosis 1, Brooklynellosis 2, Brooklynellosis 3, &  & FAQs on Brooklynellosis: Etiology/Prevention, Cures That Don't Work, Cures That Do Work, Treatment/Products/Manufacturers... & Clownfish Disease 1, Clownfish Disease FAQs 3Clownfish Disease 4, Clownfish Disease 5, Clownfish Disease 10, Clownfish Disease 12, Clownfish Disease 13, Clownfishes in General, Clownfish Identification, Clownfish Selection, Clownfish Compatibility, Clownfish Behavior, Clownfish Systems, Clownfish Feeding, Clownfishes and AnemonesBreeding ClownsParasitic Marine Tanks 1, Marine Parasitic Disease, Parasitic Reef Tanks, Cryptocaryoniasis, Marine Ich, Marine Velvet Disease, Biological Cleaners, Treating Parasitic Disease, Using Hyposalinity to Treat Parasitic Disease

Related Articles: Surviving Brooklynellosis by Mike Maddox, Clownfish Disease, Brooklynellosis, ClownfishesMaroon Clowns, Marine Parasitic Disease

...Don't eat, is in a tank corner, breathing rapidly and has some kind of whitish coat extending from the dorsal fin to the medium and lateral part of the body. Breathing hard. He has also three or four white spots on the frontal part. <All indicative> Need microscopic analysis to be definitive in your ID as other protozoan complaints are similar in appearance.

Sick Purple Tang      2/12/14
Hello,
<Jackie>
I have spent multiple days searching the web looking for some image that would answer my question, however, I have been unable to diagnose my fish with any degree of certainty.
<Looks like this Xanthurum has Cryptocaryon or Brooklynellosis; you don't see the SOP on WWM for sampling, looking under a 'scope to identify such Protozoans?>

 I have had this purple tang, Kalinda, for about four months now. She lives in a 150 gallon FOWLR system and she has been a great fish, very lively, great eater, and kind to her tank mates. A few weeks ago I decided to rearrange the rocks in the tank and take out some faux sea grass that the more timid fish liked, but that I could no longer handle looking at with all of the growth on it. After doing this, I noticed that my tang had a few white spots on her. I figured it was Ich, so I let her be and decided not to stress her out and see if she could fight it off (I will add that before I added her to this tank, my regal tang whom I had had for 8 years died in the system. The regal became ill, had white on his body, refused to eat, pretty much hung out at the bottom of the tank, and did show some signs of labored breathing.
<All classical symptoms>
Even with fresh water dips and copper,
<... have you searched, read on WWM re the use of Quinine?>

 I could not get this poor regal to recover. I figured that this might be caused by his age, but, after the outbreak I just went through, I am questioning that). After a few days, the white spots on my purple did not go away, the coverage on her didn't really look like Ich, and she stopped eating. At this point, I pulled her from the system, gave her a fresh water dip, and placed her in a hospital tank with Cupramine. I sat up with her overnight (may sound weird, but I really like my fish) and she did not show any signs of improvement and her berating became very labored. I did not think she was going to make it to morning and as I sat up all night, I continued researching and considered that she might have velvet or Brooklynella. However, looking at her and looking at the images online, I couldn't tell. She did make it through the night, but spent the night dipping to the bottom and hovering at the top for air and she seemed to be getting worse. I debated on whether or not to continue copper (for Ich or velvet) or switch to Quick Cure in case it was Brooklynella. Because my regal did not recover with copper and because my purple seemed to be getting worse, I set up a tank with Quick Cure and Stress Coat and transferred her. By the next day, she had most of her color back and her breathing was improved but she refused to eat. By the second day, she was breathing normally and she went to town on seaweed and her frozen food! It has now been five days since the crisis and she has all of her color back, her fins are healing, and she is swimming all over and eating like crazy.
So now my questions: 1) Based on the attached images, what do you think attacked her? 2) The Quick Cure said to treat the fish for three days.
<Either this or dips/baths in just the Formalin component>

I did this and I am now running carbon and Purigen through her filter. I am also doing daily water changes and have lowered the salinity in her hospital tank. Do you think I should put her through another treatment of Quick Cure at the two week mark (or four week mark - I am thinking about the life cycle of the parasite). 3) Should I treat her with any antibiotics (her tail fin did become frayed on the back edge)?
<Just the handling, copper and formalin exposure... I would not add to the med.s>
Finally, I am not sure what introduced this problem into the tank. I did place three small rocks in the refugium before the regal became ill. These were dry rocks, originally from the ocean, they sat out in the sun for some time and then I soaked them in fresh water. I also added an Allardi clown to my tank at some point.
<A ready source of/for the Brook>

I don't remember if the clown came before or after my Regal became ill but he had been in quarantine for over a month and I didn't see any signs of illness. In any case, I removed all fish from the 150 and I now have them in quarantine tanks (there are a lot of tanks in one room right now). I plan on allowing the tank to go fallow for 2 months - is this a good plan?
Is 6 weeks enough? I don't want to stress the fish out for any longer than I have to, but whatever this killer is needs to die.
Thank you in advance for your help!
Jackie Gill
180 Bow Front Reef (SPS, LPS, Softies); 4 AI Hydra LED; 2 Vortech MP40; Reef Octopus POV DC2 (downright sexy!); 36" Trigger Ruby Sump/Refugium; Apex Standard Control System. // 150 FOWLR, 2 Kessil Sky Blue LED, 3 Tunze Nanostreams, 36" Trigger Crystal Sump/Refugium; Apex Light Control System.
<... As the combo. product WITH Formalin seemed to remove the symptoms, I would read re: http://www.wetwebmedia.com/brookyescuref.htm
and the linked files above. Bob Fenner>

Tank May Have Brooklynella
Hi WWM,
<Gabe>
I have a fair amount of experience with freshwater aquarium and have decided to give saltwater a try.  My saltwater setup is a 17G Nano reef which I've cycled for four weeks using Ammonium Hydroxide.  The tank has about 5lbs of live sand and 15lbs of Liverock.  On the fifth week, the tank can process a 4ppm dose of ammonia in a little more than 24H to 0ppm (nitrites 0, nitrates <10). Figured it was time so I made a 50% water change and retested the parameters the following day, the test result was no surprise - ammonia 0, nitrite 0, nitrates <5, ph 8.3, SG 1.025, temp 84F (I'm in the tropics).
<Nice... am presently visiting in York, G.B.... Brrrr>
  Anyhoo, I got me a Yellowtail Damsel and a Yellow Watchman Goby (this was first week of  October 15).  I drip-acclimated the two for an 1.5h then introduced them to the tank, both adopted to the tank quite well.  The goby didn't come out for at least 2 days while the damsel acted like it owned the entire tank, swimming here and there in all the nooks and crannies.
<Both to be expected>
 On the third day, I went back home from work to a dead damsel.
<They are good at hiding stress...>
 Instinctively I tested the parameters - ammonia 0, nitrite 0, nitrate <10.
 I'm puzzled that a hardier fish died (the goby was fine, still is today) when all seems ok, so I dismissed it to be just bad stock that I got.  I played on the side of caution and didn't add another fish, .  However, a friend of mine had a tank emergency and needed to find a temporary home for his fishes.  Having a relatively vacant tank, I offered help.  They were all damsels, 5 of them.
<Yikes... not enough room here>
Took care of them for about a week before my friend had managed to setup a temporary tank of his own.  After that, I figured I'm ready to add stock again.  My local LFS didn't have the fishes that I wanted so I didn't bought any, but they do have a nice orange sponge that caught my eye, so I brought it home as well as 5 Nassarius snails.  Days after, the LFS phoned me that they have the stocks that I was looking for, so off I went.  I bought me, all juveniles, blue/green Chromis, spotted cardinal, and a pair of false Perculas.  This is my full stock list, I know a bit over-crowded but I'm planning to compensate this with a bit more aggressive husbandry. After acclimation, all the fishes appeared fine, same with the succeeding days, i.e. all are swimming alright, eating well, and no aggression whatsoever.  About a week in, my order for a Wavemaker arrived, it's a Resun 2000 (able to turn 2000 liters in an hour), and in it went.  I aimed it pointing towards the surface and at angle  with the internal filter's current flow.  The effect was great, to say the least, and there were no dead spots in the tank.  I've also acquired a neon-green open brain coral during this time by the way.  Two days after, the smaller of the false Percula and the Chromis, died. I was shocked because these two were doing fine all along.  I figured that they got overwhelmed by the current, but all the people I've asked with prior to me purchasing the Wavemaker, said that Clownfishes can handle a myriad of current strength from low to really turbulent ones so they should be fine with a 2000L Wavemaker. Maybe the Chromis might be different though.  
<Sometimes this genus does fine being moved, placed, other times... anomalous losses>
So, I kept a close eye on the fishes after this, then just last Sunday, I noticed something strange on the bigger Percula.  It was swimming with its dorsal and anal fins clipped to its body. 
<This is the norm for marine fishes... opposite for freshwater>
I thought, hmmm, clownfish is clowning-up.  Later that night, it was swimming close to the surface which I dismissed since it was doing this before, it did have a whitish coating on its head which I thought as just the Nassarius snails' trail that it got tangled with.
<Mmm, not this last>
 In the morning, I found it dead.  I'm devastated. The water parameters are the same as before when I tested. The cardinal, the goby, the snails, the coral, and the sponge are all doing well.  After some research, I think I might have introduced my tank to Brooklynella.
<Not uncommon>
  Meantime, I started feeding with garlic as recommended by a friend.  My question now is, do I go ahead and setup a quarantine tank and treat the cardinal and the goby with Formalin dips/baths and have them stay in the QT for the 6 weeks while I let my main tank go fishless in that entire time?
<A tough call/decision... I'd likely just hold off for now... and hope>
 Or can I just ride it out with the fishes still in? 
<Yes; this is what I'd do>
Setting up a hospital tank is not something I'm crazy doing but I guess I don't have much of a choice now, do I?  Perhaps there are better alternatives than this. Need your expert advise please.  
<There are not better alternatives... your choices are basically the two you mention>
Thank you in advance and I apologize for a very lengthy message (but you did say to be thorough with your inquiry :p)
Regards,
Gabriel M
<Do "hang in there" and keep reading for now... let's wait, see if this situation resolves/solves itself on its own. Do write back w/ developments; should you have further questions, concerns. Bob Fenner>
Re: Tank May Have Brooklynella    10/31/12

Thanks, Bob.  Yeah, to wait and see is what I've been inclined to do, hearing it from someone who's more informed than I is more reassuring though. :)  I mean, I know it would be really stressful for the fish to be put through a formalin treatment, be "hospitalized" for 6 weeks, and all that, but if I don't treat them with this regimen, wouldn't the Brook protozoa make them as hosts?
<Well, it's quite possible this isn't "Brook"... for one, the other Clown didn't contract it... Very unusual. Could be nothing pathogenic>
 Then when I add a new clown, bam! They'll hit again.  I read somewhere of a guy that had the same experience as I and that he waited 6 months without "hospitalizing" his remaining fish (non-clown) then after 6 months when he added a clown, same thing happened all over again. 
<... I would likely not add another, have two Clowns in this volume...
BobF>

Hawkfish & Brooklynella   3/20/12
Are Hawkfish known to become affected by Brooklynella? <It is normally associated with clown fish, but nearly any fish is susceptible.  Please read here, http://wetwebmedia.com/brooklynellosisart.htm > Amanda M
<Bobby> 

How to Break Brooklynella Cycle in a Tank With Several Healthy Fish     2/11/12
I have combed through your site as well as the internet at large about how to treat/cure Brooklynella, but I am still at a loss for my particular issue. I have a 72 gallon reef tank, with perfect water quality (tested often) and a list of healthy fish including a Coral Beauty Angel, Green Bird Wrasse, Cleaner Wrasse and a 6 line Wrasse. My problem is that when I put a properly quarantined fish (4 weeks) into the tank, no matter how healthy it is, within 3 days the fish is dead from Brooklynella.
<How are you sure this is the causative agent?>

 If treated and put back into the tank, it just happens again, yet the above listed member remain fine. I have performed large water changes, have a 25 watt UV sterilizer and keep my water pristine, but any addition seems to have the same fate, without fail. I am hoping there is another answer besides removing all of the fish and letting the tank remain fishless for 8 weeks while the parasite dies off. I am thinking about adding another UV unit, in hopes to eradicate the parasite that way,
<Won't>

 as my current unit does not seem to be handling it. Any insight would be greatly appreciated.
Thanks,
Ryan
<See WWM re this Protozoan, the use of quinine compounds. Bob Fenner>
Re: How to Break Brooklynella Cycle in a Tank With Several Healthy Fish     2/11/12

From all of the various diagnosis sites I have read, along with the pictures of affected fish,
<... has to be sampled, viewed under a microscope>
I am 99% sure that this is the issue, as they match exactly. It begins with heavy breathing, then mucus on the fish, pale color and then spots on the fins and body. They all also seem to seem hard against the current, seemingly to get more oxygen. From first sign of body changes, to death, is usually only 12-20 hours. Before it hits, the fish are eating and behaving normally.
How can my other fish, in particular a moderately sensitive Angel be thriving in a tank that is a death sentence for any new comers?
<Two words: "Acquired immunity"... many explanatory theories>
I will take a look at the site you sent and see if any clues can be gleaned that way.

<...>
Thanks,
Ryan
<Welcome. B>

Re: How to Break Brooklynella Cycle in a Tank With Several Healthy Fish
    2/15.12
I have read over your site for hours on end since your last email and have learned a ton, but still have an outstanding question. My tank obviously has some sort of very fast moving, killer parasite living in it and my long time fish have as you said, acquired an immunity to said parasite. So my question is, how can I rectify the tank as a whole?
<A few options... optimize/stabilize the setting... boost immunity via food supplements... and hope for the best... Removal of livestock to sufficient treatment setting, and administer medicants (quinine cpd.s most likely efficacious)... and lesser choices>
Is my only option to catch all of the fish, then dip them to kill whatever may be living on them, and then put them into a hospital tank for two months? The concept of running the tank fallow makes sense, but I fear that I will damage the corals and other inverts trying to get the fish out. I am hoping there is a better option as it seems I could be doing significant harm to my current living creatures, in exchange for the ability to add new life.
Thanks again for the help!
Ryan
<Keep reading: http://www.wetwebmedia.com/reefparasittksfaqs.htm
and the linked files above
 BobF>

Brooklynella Question    8/12/11
Hello,
<Jer>
2 months ago, I moved an 8 year old 10 gallon reef system (the only fish, a Royal Gramma died after 8 great years) and a 5 gallon reef bowl that was 1 year old into a new Modified Biocube 29. Some Lace rock was washed and added along with about 18 pounds of Live Rock as well as a mix of live and new washed sand. After a month, I added 2 Ocellaris Clownfish and 1 yellow Clown Goby. Everyone was happy and healthy until 2 days later, I awoke to find that one of the clowns had jumped into the back chamber of the Biocube and died (I built a Plexi glass divider to keep this from happening in the future).
<Ah good>
The LFS that I have shopped at for almost a decade was nice enough to give me a replacement. The replacement fish seemed pale when I got it home (I figured from stress), but I floated and dripped it for a while, then released it into the tank. Everyone seemed to get along well, and the new fish ate some frozen brine and flake food. The next morning, the new fish was dead and stuck to the powerhead. He seemed to have a thin white coating on him, but was eating really well the day before he perished. I removed the fish and returned him to the LFS for store credit and I wasn't about to introduce another fish into the system until I figured out what was going on. I just noticed that my original clown that had been in the tank is now starting to turn dark/black in the area around his dorsal fin and it seems like there is a thin white substance in that area (noticed this 5 days ago). It doesn't seem to be spreading, and he eats great, what do you think this could be?
<Can't tell>
Could this be the onset of Brooklynella or does it move faster than this?
<Almost always yes>
I've tried to snap pictures, but my digital camera can't seem to take a good photo of him. I'm going to try and create room for a QT tank to introduce new fish, coral, etc into before putting them in my display, but in an apartment, it is tough to find the room. Any advice other than getting a good photo?
<Read: http://wetwebmedia.com/brooklynellosisart.htm and the linked files above>
My tank parameters are: 1.023 SG, 79-80 degrees, PH 8.2, ammonia 0, Nitrite 0,Nitrate 0 (all of these were confirmed by my tests as well as the LFS tests. Should I be testing for Alk?
<Possibly... IF you are in the habit of using the same salt mix brand, regular water changes, not likely a routine you'll have to repeat consistently>
Thank you!
<Welcome. Bob Fenner>
Jeremiah
Thanks, I'll try to get a photo of the clown so you can see what I'm seeing
Re: Brooklynella Question    8/12/11
I was able to snap a photo. Please see attached. Thank you!
<Sure looks like Brook to me. Have to look at a skin mucus smear under a 'scope to be sure... B>

Re: Brooklynella Question    8/12/11
Thanks for your opinion. I should pull the fish out and do a dip in?
:<Disappointing... READ where you were initially referred>
If so, a dip in what? I don't have a qt tank, so should I return the fish to the tank after treatment? Or should I set up a new 5 gallon bucket with heater and air stone as a makeshift qt tank?

Brooklynella... no, not likely   1/5/10
Hello Crew,
<Matt>
I have recently had a major outbreak of Brooklynella in my tank. It killed 2 Yellow Tail Damsels,
<Mmm, doubtful>
2 Clowns, and a Royal Gramma. What I have left are a Haitian Anemone, Scooter Blenny, 2 Camel Shrimp and a few snails. What I'm asking is how long should I wait before I start adding new fish back into my tank? Thank
you for your time.
<... This is/was much more likely some other Protozoan... Probably Cryptocaryon... Read here: http://www.wetwebmedia.com/ichartmar.htm
and the linked files above... till you understand your situation. Bob Fenner>
Re: Brooklynella 1/6/10
Thank you for the quick response. I still do not understand how long I should wait before adding any fish to my tank.
<... Need to make sure this is/was a parasitic infestation...>
The reason I thought it was Brooklynella was because all of them became white, Not spotted but in like a white carpet.
<I see>
And died within a day. I'll continue to read that link you gave me but any extra information would be very helpful. Thank you.
<I would likely treat all with a quinine compound (will cure both Crypt and Brooklynella)... In a separate treatment tank... leave the main/display fallow (sans fish hosts) for at least four weeks... Read here: http://wetwebmedia.com/quinmedfaqs.htm
and here:
http://wetwebmedia.com/brookyescuref.htm
and where you lead yourself otherwise. Bob Fenner>

Clownfish recovering from Brooklynella without treatment  11/13/09
First off thanks so much for maintaining such a great resource.
It has been a big help to me! I mostly use the web to research on my own and don't post much but I need to some help on something I can't seem to find an answer for.
<Yay!>
I recently lost my male Black & White clown to what I think was Brooklynella. Now I am not so sure if it was Brook or not. I have had these clowns since Feb 2009 without a single issue.
<Mmm, if no new Clownfish or fish period had been introduced in recent time (w/in weeks) this is doubtful. More likely some other parasite or even "just" environmental effect>
The male had all the symptoms of Brook (white mucus coating the body, head shakes, darting, not eating, enlarged gills, heavy breathing, trying to stay in the current). He died within 24 hours of the mucus showing up (5 days ago). The strange thing is that the Female is still in the tank today and is the only fish currently in the tank (40 breeder). The day after the male died, she started to develop a partial white mucus coating but she did eat quite well that same day. I have also seen her being routinely groomed by my cleaner shrimp which I have never seen her do prior. She has eaten very well every day this week so far and last night the mucus was limited to only one area on her lower body (which the cleaner shrimp was tending to later). She truly looks really good now but she was still darting a bit last night.
When I fed her tonight she ate more food and more aggressively than I have ever seen her eat.
<Good signs>
From everything I read (and saw with my male), I don't see how she could be on the road to recovery without treatment if this was Brook as it attacks very quickly.
<I agree>
When the male died, I went out and bought a hospital tank for the female but I have not set it up because she is doing so well and I don't want to stress her if it isn't necessary.
So here is my question...IF she makes what appears to be a full recovery without treatment, will I be able to add more fish in the future without taking her out and treating?
<Should be, yes. You may have a resident parasitic fauna (most systems do), that could conceivably become (hyper-) infective at some point, given circumstances that promote it over the fishes' resistance>
I am in no hurry to add another fish and have already committed to waiting a couple of months at least to add any fish whether I have to treat her or not. I have tried to find an answer to this question on my own but I did not have any luck.
Current parameters: Ammonia 0, Nitrate 0, pH 8.0-8.1, SG 1.025 (no Nitrite kit). No skimmer or refugium, 30lbs of live rock. I do weekly 10-15% WC's and I have not had any variation to these numbers that I am aware of.
Also, I would like to add another clown for her to hopefully pair up with. Is the "purchase a smaller one and he'll remain the male" accurate or are there no guarantees?
<This general statement does apply. Do buy a smaller individual. About an inch in length maximum>
Thanks,
Graham
<Welcome! Bob Fenner>

Brooklynella question   9/9/09
Hello there,
<Brett>
I've been reading your question/answer emails about fish disease and I wanted to get your take on a problem that I'm having. First, some background info: I have a 55 gallon tank with a 10 gallon sump/fuge. It is a reef tank and I have several soft corals as well as some LPS. As for lighting I currently have 4 x 65 watt Coralife PCs, but I will be upgrading this week to a Nova Extreme 8 x 65 watt T5 fixture. Probably about 70 lbs of live rock and a 2 inch sand-bed. I have a Remora hang-on skimmer and some Chaeto and mangroves in the fuge. Fish: 1 baby hippo tang,
<Needs more room than this>
3 blue damsels, and 1 ocellaris clown.
My water parameters: Ammonia: 0ppm, Nitrite 0ppm, Nitrate 0ppm, Phosphate: 1ppm,
<1.0? Way too high>
Mg: 1250, Ca: 460, Alk: 4.5 mEq/mL. SG: 1.024, temp: 78. I have had the tank running about 2 months now (I'm sure the nitrates will start to climb soon). I use RO water and Instant Ocean salt mix.
When I first got the tank I got two young ocellaris clowns. Both died within a week. I didn't QT them, unfortunately. I didn't notice any sloughing-off of the skin or any spotting, but they stopped eating, became much more inactive and gasped a lot near the bottom of the tank. I added the hippo tang and the damsels between the deaths of the first clown and the second, also without QTing.
At this point, determined to keep some clowns...I set up a QT with a heater, airstone, PVC and some ammo-lock on the side just in case.
<Not a good method of handling ammonia here>
I bought two adult ocellaris clowns and put them in the QT. They did great in there for two weeks and then I put them in the display. After 1 day, one of them looked all powdery. I immediately thought Brooklynella. The other one looked fine.
<Mmmm, unusual for one to express and not the other>
I gave the spotted one a freshwater bath and then put him back in QT. I also put the other one in QT as well, figuring that he just wasn't showing symptoms yet. I treated the QT with Cupramine, just in case it was ich and because I had not been able to track down any formalin until today.
<... what about your other fishes?>
That was last night when I returned them to the QT...this morning one was dead. I couldn't see any "white powder" on either the dead fish or the remaining one, but I'm assuming that the white-dusted fish had been the victim. Today my girlfriend is picking up the formalin and is going to treat according to the directions on the bottle in the QT.
<Do take care here: Read: http://wetwebmedia.com/formalinart.htm>
Here's my question: It seems as though the display tank is infected with Brooklynella,
<Seems? W/o looking at a skin/slime scraping through a 'scope, I won't agree>
probably from those very first clowns that I put in. I'm not going to return the clown that I'm treating with formalin to that tank (if he survives the treatment and QT). I'm also not planning on taking out the damsels or the tang. The tang has not shown any spots or sloughing...he is still a brilliant blue color. He has been in there for over 1.5 months since the first set of clowns died. At one point he did start scratching on the rocks a lot, but it seems that if I feed him garlic extreme soaked food that he becomes fine again and doesn't scratch. Is he acting as a host for the Brooklynella?
<Possibly>
Is it okay to leave him in the display since he has survived for 1.5 months and seems to be doing as well as ever?
<Your call>
I could try to QT him too, but he is very hard to catch.
Also, if I do try to reintroduce the clown after treatment, how long should I wait?
<A month or more>
I read on one of your answers that unless you completely empty and then bleach the tank that the Brooklynella could stay in it indefinitely. That's not an option for me. I was thinking of maybe waiting 6 weeks. If the clown gets symptoms again then, I guess I will just give up on keeping clowns in this tank, unfortunately. What should I do about the tang though? Is it possible that he's immune to whatever's in the water (presumably Brooklynella)?
<Some Surgeonfishes are known to be susceptible... but I don't think this is/was Brooklynellosis... if it were, all Clowns would have been afflicted>
Thanks in advance!
<I would return the one adult Clown, wait the six weeks and purchase a couple of tank bred specimens and place them. Bob Fenner>

Shrimp goby pair quarantine... Actually, Disease, part. Protozoan ID, trtmt.   8/17/08 Hello to all and thank you for being able to ask questions, <I as well> it is greatly appreciated. I am in need of some guidance and direction as to what is the best way to address my current situation. My tank came down with what I now believe was Brooklynella. <Mmmm, not likely> In one day I thought I lost all fish. <Then no> Symptoms were cloudy eyes, peeling skin and 2 dead fish clown and pseudo. I also had a yellow watchman goby pistol shrimp pair which I had assumed perished as well because I had not seen them in a while. I was going to let the tank go fallow following directions listed on your site. Then one day I put a very small amount of frozen food in the tank and out comes the goby and shrimp looking very healthy. So my question is now what do I do? <Likely nothing> Im thinking of dismantling the tank to catch the goby and shrimp and place them in a 10G quarantine tank. <... for what reason/s?> Should I catch both or just the fish? If just the fish will they still pair off later after reintroduction? Also what should I use to treat the yellow watchman goby and how often since I see no signs of disease on him? <I would do none of the above> I was planning on using a sponge from my tank and also water from the main tank into the quarantine, but would it be better to not use the water from the main tank since the disease is in the water from the main tank? I would really like to keep the goby paired up with the shrimp but since I will most likely be medicating the fish I am unclear as to what to do? If you recommend a dip and then placing in quarantine how would I know if it worked since I don't see any signs of disease on him and its been 10 days now since I lost the other 2 fish? Thanks Steve Tank size is 24 gallons water quality ph 8.2 ammonia 0 nitrite 0 nitrate <5 specific gravity 1.025 refugium in the process of being installed 20% water changes weekly 22 lbs live rock 1/2 in sand various zoos 1 blue xenia <Mmm... let's start a bit toward the beginning here... W/o knowing... what the actual cause of loss was here I would not "jump" to conclusions... was this a protozoan infestation? Not definitively... NOT Brooklynellosis I assure you. I would leave all as they are presently... proceed cautiously in introducing new fish livestock (see WWM re... quarantining, assuring the initial health of new introductions). Bob Fenner>

Brooklynella Parasite Help! Just reading...  07/07/08 Yesterday morning I woke up and found my Tennent Tang was sideways in the tank "flopping" around. <Yikes!> I immediately ran to a pet store I trust and they told me that the fish would die and if I wanted to, I could bring him in and have a biopsy. When I arrived home, my tang was dead. I drove the drive back to the store and they found that my fish had died. <?> They scraped the sides and found a Brooklynella Parasite and told me that I could not do much, as I have many invertebrates in my tank. <... Where did this Protozoan come from?> I was told to gradually lower the salinity to 1.018 and gradually raise the temperature to 83 degrees (each degree and amount of salinity in 24-48 hours) - but to closely watch that everything isn't getting to disturbed in the meantime. Please help me if you have any experience in what to do with this parasite when housing invertebrates with fish (other than what I was told). I am desperate to get everything healthy again and not to loose anything more! Thank you! <... I am desperate to have you read here: http://wetwebmedia.com/brooklynellosisart.htm and the linked files above. Bob Fenner>

Re: Brooklynella Parasite Help! Still...  07/07/08 From message question below - we do not know where it came from. <... read> I have had this tank for over a year and the most recent thing I have purchased for the tank was months and months ago (a decorator crab). The person at the pet store said he thought it had been infected for a week or so, <...?> from looking at its biopsy. I just don't know what to do - I read your link, but it still doesn't help me with what I should do to cure this seeing as I have Invertebrates in my tank. I'm just upset because that Tennent Tang was such a good "buddy" of mine. I just want to be able to save everyone else. Again, I appreciate your help with this! <... keep reading. RMF>

Possible Brooklynellosis... -- 03/07/08 Hi Crew, <Pat> Long story short. somehow velvet made it way to my DT and I QTed all the fish. My tank is now fallow. I FW dipped my two clown fish for ~10 min, QTed and Rx with copper as directed <Uhh, copper won't cure Brook...> and I am at day 19 and no more signs of velvet. BUT I noticed about 5 small white spots with a little protrusion on the female today and can only think that they now are showing signs of Brooklynellosis since they have been in copper for so long. <Mmm, may be just environmental... reaction to the copper poisoning> None of the other fish in the other QT tanks show signs. I could not find any information on the cycle of this protozoan. <Is very similar to Crypt...> Will leaving my DT fallow for 6 weeks rid my system of it? <Hopefully, yes> What is the best way to deal with this problem, since a FW dip did not take care of it would a formalin dip or formalin medicated tank do any better? <Yes... Read here: http://wetwebmedia.com/brooklynellosisart.htm and the linked files above> Could the other fish also have it, what should I do with them, keep in QT and monitor for another 2 weeks? <I would, yes> Also, I do not have formalin on hand and if I can not get it locally should I at least do a FW dip to help remove them until I can get some? <If the fish appear to be suffering, likely worth the wear and tear...> Thanks, Patrick <I would NOT be so sure this IS a pathogenic manifestation... Do read where you are referred to. Bob Fenner>

Brooklynella??? Please help!!!   6/9/06 Hi WWM crew, I have an emergency...unless you can put me at ease somehow.  I have a captive bred clownfish that I have had for over a year now.  He is in a 5 month old 95 gallon tank with 100lbs of live rock and live sand.  Water parameters are great.  Ammonia 0, Nitrites 0, Nitrates <20, pH 8.3, salinity in between 1.023-1.024, temp steady at 78F.  His tankmates are two Pajama clowns that have been there for 4 months and all have been perfectly healthy. My clown has a cotton like patch on his left side, just at the lateral line and past his dorsal fin.  It does protrude off of him somewhat, but is difficult to get pics or more accurate description because he simply will not stay still.  He is eating well.  He is not, nor has not been near the top of the water gasping, neither is he lethargic at all.  He still swims actively around his two anemones <This may be "it"> and his fins are not clamped.  I don't know what a fish healing from a scrape looks like, or I could assume that... if it is a scrape, then it's a nice one.  His respiration seems normal, but it's hard to tell as he swims constantly.  The other fish show no signs of anything thus far. Would the fish still be eating if it were Brooklynella? <Perhaps, but not with gusto for long>   I read on this site and the section on Brooklynella thoroughly and couldn't find an answer to that question.  If it were advanced enough to be on his body would he still be acting fine? <Is not this protozoan unless you have introduced it somehow recently...>   He does twitch and dart some, but I haven't noticed any flashing.  If he flashes, it isn't often... but he does twitch like something is biting him nonetheless.  I read that it seemingly starts at the gills.  If it is in his gills, would he still be eating and swimming in the middle of the water column?  Please, any info or advice that you can give would be very welcome and appreciated!!!    Thanks again, H. Owens <I wouldn't move, treat this specimen. This is highly unlikely to be a parasitic involvement. Could be a negative interaction twixt the anemones, but much more likely resultant from a physical trauma as you speculate. Bob Fenner>
Re: Brooklynella??? Please help!!!   6/10/06
Hi again and thanks for the super quick reply!  If this is  Brooklynella, approximately how long will fish normally continue to eat after the  "bug" shows up on the fish? <Impossible to say, however, this protozoan is generally very rapid "on-set", killing its host/s within days of discovery...> The spot appeared on Tuesday and as  of Thursday night, he was still eating like a pig. <... is almost assuredly not Brooklynellosis...>   I only put two new  pieces of live rock in the tank over a week ago after holding it for over a  week.  The tank came from the LFS that has a live rock only tank with 1  Naso tang in it.  It had been in his tank for some time.  Is it  possible that it could have been introduced that way?  I guess, my main  concern is, would he still be eating if it was far enough along to be showing  up on his body?  Thanks again for your help! <I wouldn't be concerned... please read: http://www.wetwebmedia.com/clndisfaqs.htm and the linked files above. Bob Fenner>

Clownfish Breathing Hard hello I have a clown fish that is breathing real hard but the weird thing is that everything in the test kit that I have is all perfect? what do you think it is and what should I do for it? thanks <Many possibilities here... could be just simple anoxia (lack of aeration, circulation)... possibly an osmotic imbalance (too much change in your water make-up in too short a period of time), perhaps a Brooklynella infestation... Please read through the Clownfish Health/Disease sections and related FAQs files posted on the site: www.WetWebMedia.com for hopefully what will surface as the cause/s, path to take here. Bob Fenner>

Brooklynella, Microsporidium, or Lymphocystis? Hello, I recently made the mistake of purchasing a large (3") Amphiprion Ocellaris (common clownfish) from my local fish store. They had just received the animal when I purchased it, mistake number two. I added the clownfish to my newly cycled reef tank (mistake number three, biggest one). Within 24 hours I noticed a white wart/cauliflower growth on the animal's right side, in the white band area, just behind the gills. The clownfish will not eat and appears to be stagnant although it is moving about somewhat. The animal does not exhibit any other signs of infection, no small white spots, glazed or popping eyes, scratching, fin rot, and the like. What has infected my fish? I've narrowed it down to either Brooklynella, Microsporidium, or Lymphocystis or ? ? ? What can I do to cure the fish and save my reef tank. Should I remove the infected fish to prevent the disease from spreading to the other tank mates (choc chip star, scooter blenny, 2 turbo snails, 6 reef crabs)? Please advise, I want to save my tank and the clown if possible. thanks, Dan Rose >> Thank you for writing so well, including with such clarity and humility... Glad to know that I'm not the only one who makes egregious errors in the hobby... I would almost bet that what you are seeing is indeed the Clown-attacking protozoan Brooklynella... And would in any case follow these steps. Do take the fish out and treat it in a separate "hospital" system... for it's sake, the use of the treatment "medicine", and the safety from the same of the rest of your system. The likelihood that the Brooklynella will affect your other livestock is minimal (there are no other Clownfishes...)... Copper is of little use if indeed this is Brooklynella, but formalin dips/baths are efficacious. Buy a stock solution (my fave retail brand is Kordon, but any will do)... generally a few drops per quart of pH adjusted (just with sodium bicarbonate, baking soda is fine) freshwater... and move the fish from the main tank into this solution for about ten minutes... either add an airstone, occasionally splash the water around for aeration), move the Clown to the bare-bottom (but still filtered, aerated.... with a hang on, sponge, box filter...) hospital/quarantine system... Dump the treatment water... repeat every day for three days... and stop there if there is no further visible sign of the disease... If it re-shows or persists, wait two days and resume the dip/baths... This will work....  Bob Fenner 

Ich freaking me out what are these spots still on my fish today, slime kinda <likely a protozoan infection so common on clownfish... do read more about it and treatments (which you are already doing) at http://www.wetwebmedia.com/parasiti.htm Let me encourage you to use the wealth of information in the WWM archives (articles, FAQs, photos and more) and use the Google search engine on the WWM to narrow your search if necessary (I prefer to just browse). Best regards, Anthony> <Looks like Brooklynellosis to me... Please see the Google search feature on WWM re "Brooklynellosis" and/or the FAQs files on Clownfish Disease: starting here: http://www.wetwebmedia.com/clndisfaqs.htm and beyond. Need to act, quick. Bob Fenner>

Ocellaris Clown (disease) Hello Sirs. Thank you again for all of the info. I'm writing to get your opinion on diagnosis/treatment of my false percula. After searching the site, I found a post that seemed pretty similar to my situation (as far as the symptoms). It goes:   Brooklynella, Microsporidium, or Lymphocystis? Hello, I recently made the mistake of purchasing a large (3") Amphiprion Ocellaris (common clownfish) from my local fish store. They had just received the animal when I purchased it, mistake number two. I added the clownfish to my newly cycled reef tank (mistake number three, biggest one). Within 24 hours I noticed a white wart/cauliflower growth on the animal's right side, in the white band area, just behind the gills. The clownfish will not eat and appears to be stagnant although it is moving about somewhat. The animal does not exhibit any other signs of infection, no small white spots, glazed or popping eyes, scratching, fin rot, and the like. What has infected my fish? I've narrowed it down to either Brooklynella, Microsporidium, or Lymphocystis or ? ? ? What can I do to cure the fish and save my reef tank. Should I remove the infected fish to prevent the disease from spreading to the other tank mates (choc chip star, scooter blenny, 2 turbo snails, 6 reef crabs)? Please advise, I want to save my tank and the clown if possible. thanks, Dan Rose Thank you for writing so well, including with such clarity and humility... Glad to know that I'm not the only one who makes egregious errors in the hobby... I would almost bet that what you are seeing is indeed the Clown-attacking protozoan Brooklynella... And would in any case follow these steps. Do take the fish out and treat it in a separate "hospital" system... for it's sake, the use of the treatment "medicine", and the safety from the same of the rest of your system. The likelihood that the Brooklynella will affect your other livestock is minimal (there are no other Clownfishes...)... Copper is of little use if indeed this is Brooklynella, but formalin dips/baths are efficacious. Buy a stock solution (my fave retail brand is Kordon, but any will do)... generally a few drops per quart of pH adjusted (just with sodium bicarbonate, baking soda is fine) freshwater... and move the fish from the main tank into this solution for about ten minutes... either add an airstone, occasionally splash the water around for aeration), move the Clown to the bare-bottom (but still filtered, aerated.... with a hang on, sponge, box filter...) hospital/quarantine system... Dump the treatment water... repeat every day for three days... and stop there if there is no further visible sign of the disease... If it re-shows or persists, wait two days and resume the dip/baths... This will work....  Bob Fenner Well I thought that the "wart/white cauliflower" part definitely defined what has appeared on the very tip of the dorsal fin of my fish. I've had this fish about a month. 2 weeks in QT and 2 in main display. About 3 days after introduction into the main tank, I noticed a white spot about the size/shape of a grain of table sea salt (yum). Wasn't sure so I've been watching and reading in the meantime. In the last 10 days the spot has maybe grown by 25-50%, but has not spread to any other areas. It also has not spread to Pac Blue Tang. He (or she I'd suppose) was eating normally until yesterday when he/she started "sampling" then spitting out food (similar CMA recipe). Seems similar enough to enclosed post to warrant an email. Would this be a Brooklynella infection that would require the above recommended treatment? <Mmm, the wart-like growth on the Clown could be lymph, the spot on both fishes is likely crypt, tangs don't "get" Brooklynella> Other info: 125 gal, 90# LR (more coming when the $$ does), 200# LS, 240W NO fluor. lighting @13 hrs/day (MH with more $$ again), 2x Rio 1100 plus return for circulation, TF 1000 skimmer, sump w/ 55W PC lit 'fuge, airstone and active carbon. Today's parameters were pH-8.2, Ammonia-0, Nitrate- <5 ppm, Alk- 4.8 mEq/L, Ca-350 ppm, Temp 77, SG-1.025 Other livestock: 1- Pacific Blue Tang, 1-sand sifting star, 12 bumblebee snails(?), 6-turbos, 12-astrea, 1- hitchhiker clam as of yet un-i.d.'d, misc hitchhiker polyps/sponges etc, and very soon 1- cleaner shrimp to be determined <I would hasten the acquisition of the Cleaner Shrimp/s... maybe get more than one> Hope this is enough/ not too much info. Thank you for your help and dedication to helping the rest of us become as informed as we can possibly be. <Perhaps add a Gobiosoma Goby to the Cleaner list as well... otherwise nothing "stands out" as real trouble/cause here. Bob Fenner>

Beating Brooklynella! Hello guys! I am new here so I hope you would help me... and quick if that's not too much to ask? <Scott F. at your service!> This morning, I noticed that my Flagfin angel developed some white patches all over his body. Though I didn't notice any adverse reactions from this, I opted to do a 10 minute freshwater dip just to be sure. I know this is something serious because from hindsight, a couple of really healthy bi-color angels I had in the past developed similar symptoms where their entire body turns hazy, almost white and then dying a day later. <Not good...> I know this is not ich because there are no apparent white spots. I also discount the fact that this might be velvet because the fish doesn't exhibit rapid breathing, is not flashing and he is still feeding. <Well, the fact that the fish is eating is a very encouraging sign...> Same thing in the past with my bicolors. My best guess is that this might be Brooklynellosis. I read from your site that this occurs mostly among Clownfishes, but what do you think? How can I cure it? I'm not considering formalin because I have no access to it. Will hyposalinity work? <Well, it might provide some temporary relief from symptoms, but it is not generally considered a "cure" for Brooklynella. As you point out Formalin-based medications, and Malachite Green meds are usually considered the proper cure for this illness. Feeding the fish with antibiotic-laced medication after they are healed can help prevent the occurrence of secondary infections in these fishes.> I also put a really nice Centropyge flavicauda yesterday before I noticed the disease on my Flagfin. What are the chances that this fish might contract the disease? <It's really hard to say...This is a highly contagious illness, and I'd operate on the assumption that any fish which has been exposed to it is a potential victim...I'd remove all fishes from this tank (even the apparently healthy ones) and place them in a separate quarantine tank for observation and/or treatment if symptoms manifest. Meanwhile, you'd let the display tank run "fallow", without fishes, for about a month. This will result in a significant decrease in the population of the Protozoans that cause this disease. Not a fun procedure, but quite effective> What can I do to prevent it from attaching to this fish? <As above> I also have a whitecheek tang, clown tang and 2 Ocellaris clowns in with these angels, though I don't seem to notice the parasites attaching to them... ever! Why could this be? <Hard to say...Could be some form of resistance- could just be random luck...It's up to you if you want to approach the management of this disease aggressively, or wait it out and see...> I'm planning to do a 25% water change tomorrow to help alleviate the situation. <A water change is always a good thing. Not sure, however, if it will have any impact on this illness in a display tank while fishes are still present> Pardon me for the long story, just like to divulge all important details. <You did a great job! No problem!> I will greatly appreciate your response! Salamat nang marami, from your friend here in Manila!  Regards, Mitch <Best of luck to you, Mitch! I hope that things work out okay! Good luck! Regards, Scott F>

- Tomato Clown Disease? - Hi Jason (or whomever is kindly helping me here), <It's Jason again...> Spots have now spread across both sides of body & fins and seem to be both black and white. Have attached 2 more photos (which I hope will go through).  <Got them... interesting.> Am not sure of a plan of action here and am simply trying to rule things out at this point so that I don't start a treatment plan that ends up being more harmful for the fish than the problem.  <Good tact...> Of the common diseases that I've read about I can't seem to identify this as being any one of them because of the following reasons: Amyloodinium - can't be this because the fish has had problem for a while (3 or so weeks) and has exhibited no signs of breathing difficulty. Seems like this disease doesn't show any skin symptoms (except for 2nd hand diseases).  <Ok.> Cryptocaryon (Ich) - thought it might be this, but the spots are hardly 'salt' like and the other fish in the tank (a scooter blenny) has not shown any symptoms at all.  <Hmmm... not uncommon for one individual's stress to be high enough to suffer from a parasitic problem like this before other fish in the tank.> Brooklynella - Local dealer told me that it was probably this after I described the symptoms (& sold me some Formalin to use in a 30min dip), but I really doubt it is this as it looks nothing like the pictures I've seen of it & furthermore, the fish again doesn't exhibit the 'gasping' & reduced appetite that I've read about. Finally, it is my understanding that Brooklynella (like Amyloodinium) would probably kill the fish rather quickly. As I've mentioned before, the fish seems to be otherwise doing fine... So, I have CopperSafe ready to go, Formalin ready to go & am ready to use these medicines either in a quarantine tank (Copper) or as a dip (Formalin), but as I don't really know what I'm supposed to be treating, I don't know what I should do. I looked for Methylene Blue at the store & wasn't able to find it (is it sold with a specific brand name?) but will use it in a buffered freshwater dip if you feel it would help.  <The dip is a good way to kick things off. Perhaps the isolation would also be helpful here.> Anyways - any identification you could give me? <Still not sure, and really looks to me like aggression, nipping... or scraping against something. Will do some more research.>   If not, any ideas on a plan of attack?  <I like the idea of a dip along with isolation in quarantine to await further action.> Hope these photos are helpful... <Yes.> Thanks again for the help in advance. Know you guys are busy... Eric Harvey <Cheers, J -- >

 

URGENT_ sick clownfish: Brooklynella 5/21/04 Hello, <howdy> When I woke up to turn on the tank light I noticed that one of my percula clownfish was very badly injured. He has this mucus white cobweb like stuff all over him and is breathing hard. I don't know what is wrong.  <This sounds like classic "clownfish disease" (Brooklynella... do a keyword search of this term on our website. Google search tool on the home page)> The female is fine and is eating, but I noticed her nipping at her mate. I believe that the female is pregnant because she is quite large (stomach area), and this is leading to stress. Nothing appears to be wrong with the other tank mates. My tank is 75 gallon, live rock, skimmer, UV, filter, liver rock, 9 months old. pleas help I don't want to lose him. Scott <the disease generally only afflict new fish or comes in with livestock recently added to the tank without quarantine. It is critical to QT all new livestock for 4 weeks without exception (all new fishes, corals, rock, snails, plants, shrimp, crabs... everything!). Else you risk random infections perpetually. Treatment of Brooklynella generally requires Formalin. The Aquarium Products brand "Quick Cure" may work here with aggressive freshwater dips. Do see our archives for more details on how to do this. Anthony> 

Clownfish nodules related to Brooklynella? Hello there! I recently moved my pair of 3/4" ORA tank-raised Ocellaris out of their three-week quarantine and into my tank. They did not appear to be afflicted with anything, and ate just fine previously.  My question really only pertains to one of the clowns, the one that is developing larger white nodules. By nodules, I mean what seem to be larger, semi-translucent fluid-filled sacs, anywhere from 1mm-4mm in diameter. The nodules seem to stick to her (the larger clown) at certain areas, but most prominently the gill covers and tail fin. They are occasionally found elsewhere also. The fish is fine as far as activity goes, and eats like a pig. Are these nodules in any way related to Brooklynella? <Don't think so... have battled with this protozoan, dissected many clowns...> I'm simply afraid that although they are the only two fish in the system, that I may have moved them through my quarantine procedure without being cautious enough. Can you help me? <A bit at least... I strongly suspect this is NOT a pathogenic reaction... the folks at ORA would not have such in their systems...> Thanks so much for all the time and effort you put in to answer the questions that fellow hobbyists such as myself have. Quentin D. <I wish I could tell you more... is very strange that only one of the two is affected... I do hope the blistering will fade of its own accord. Bob Fenner> 

Brooklynella   12/28/05 To the WWM Crew <Flávio> In a small, 20 Lts., quarantine tank I have a clownfish probably with Brooklynella. He arrived 4 days ago. <Am sure you're beginning to appreciate the value of captive produced clowns versus wild-collected...> Don't eat, is in a tank corner, breathing rapidly and has some kind of whitish coat extending from the dorsal fin to the medium and lateral part of the body. He has also three or four white spots on the frontal part. <All indicative> Today I added Coppersafe, one cupful as the manufacturer indicates. In the meantime I read your advice about Brooklynella and dips in formalin ( 15 to 30 minutes with 1ml (20 drops) for 4 Ltr of salt water, as you state). <Yes... if Brooklynellosis, copper will not cure it> My question is if I can put some formalin in the quarantine tank in order to treat the possible occurrence of the two diseases. How much formalin could I put there. 2 drops? Thanks in advance Flávio <I would not place formalin in your quarantine system if you can treat otherwise... instead I would run a dip/bath and move the animal to a newly cleaned/sterilized system to avoid cross-contamination. Please see WWM re Formalin use, http://www.wetwebmedia.com/formalinart.htm, Brooklynella. http://www.wetwebmedia.com/brooklynellosisart.htm Bob Fenner> 

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